Please note: In 2003, the CTF updated its Grades of Recommendations to include an "I Recommendation" for situations where insufficient evidence exists to allow a recommendation to be made.  (Formerly, these situations were captured under a "C Recommendation".)  This change is not retroactive, and all "C Recommendations" made prior to 2003 have not been reevaluated in light of the new "I" recommendation grade.  For a discussion of these recommendation grades, please link to the 2003 article in the Canadian Medical Association Journal here.

Preventive Health Care, 2000 Update: Screening and Management of Hyperhomocysteinemia for the Prevention of Coronary Artery Disease Events

Prepared by Gillian L. Booth, MD, Departments of Medicine, Clinical Epidemiology and Health Care Research Program, University of Toronto, Elaine E. L. Wang, MD, Departments of Pediatrics, Clinical Epidemiology and Health Care Research Program, University of Toronto

Objective
To evaluate the quality of evidence pertaining to homocysteine and coronary artery disease (CAD) and make recommendations regarding screening and treatment of hyperhomocysteinemia.

Burden of Suffering
Cardiovascular disease is the leading cause of death in Canada, accounting for almost 40% of all deaths. While mortality rates for ischemic heart disease are declining, the costs to society remain high. Since a number of cardiovascular deaths may be preventable, the search for novel risk factors continues. Homocysteine is an intermediate that is generated in the metabolism of methionine. Therefore, altered homocysteine metabolism has become the focus of increasing attention based on its potential role in the pathogenesis of atherosclerosis and other conditions, such as venous thrombosis and neural tube defects.

The prevalence of hyperhomocysteinemia in the general population is between 5 and 10%, based on a threshold set at the 90th or 95th percentile (approximately 15 mmol/L). However, rates may be as high as 30 to 40% in the elderly. If population-based studies are correct, then up to 10% of coronary events may be attributable to plasma homocysteine. Thus, homocysteine may represent an important and potentially modifiable risk factor for cardiovascular disease.

Options
Screening of serum homocysteine in patients who have either no symptoms of CAD at baseline (primary prevention) or those with known CAD (secondary prevention); treatment of patients with high homocysteine levels to prevent CAD

Outcomes
Cardiovascular death and overall mortality in patients with established coronary artery disease.

Evidence
MEDLINE was searched from 1966 to June 1999 using the MeSH headings homocysteine; hyperhomocysteinemia; methionine; coronary disease; arteriosclerosis; myocardial ischemia; folic acid; vitamin B12; vitamin B6; and pyridoxine.  Relevant articles were also identified through a manual review of references.

Values
The 10-member Task Force of experts in family medicine, geriatric medicine, pediatrics, psychiatry and epidemiology used an evidence-based method for evaluating the effectiveness of preventive health care interventions. Recommendations were not based on cost-effectiveness of options. Patient preferences were not discussed.

Background papers providing critical appraisal of the evidence and tentative recommendations prepared by the chapter author were pre-circulated to the members. Evidence for this topic was presented and deliberated upon in a 2-day meeting in May 1998. Consensus was reached on final recommendations.

Benefits, Harms, and Costs
High-pressure liquid chromatography (HPLC), the most common method used to measure total homocysteine (tHcy), has a coefficient of variation of 3 to 11%.  tHcy levels may be falsely lowered in the acute phase of illness, such as myocardial infarction, while factors that may elevate tHcy include genetic predisposition, increasing age, male gender, serum creatinine, as well as delays in placing samples on ice.  Medications such as anti-epileptic drugs, methotrexate, nitrous oxide, and certain disease states, such as psoriasis, acute lymphoblastic leukemia, breast cancer, and hypothyroidism also increase levels, likely through effects on vitamin status.  Homocysteine is inversely correlated with serum vitamin B6, B12, and folate.  Thus, in populations with a higher prevalence of B12 deficiency (such as the elderly), the specificity of plasma tHcy as a cardiac risk factor may be reduced.

Current costs range between $30 and $50.  However, newer, less costly techniques for measuring tHcy have been developed and should become more readily available.

Recommendations
Recommendation grade [A, B, C, D, E]  and level of evidence [I, II-1, II-2, II-3, III] are indicated after each recommendation. Citations in support of individual recommendations are identified in the guideline text.

Validation
This report was externally peer reviewed.  The American Heart Association state that it may be reasonable to screen individuals who are at risk for hyperhomocysteinemia (such as patients with renal failure) or those who have a personal or family history for premature atherosclerosis.  Several experts in the area concur.

Sponsors
The Canadian Task Force on Preventive Health Care developed this guideline with funding from the Provincial and Territorial Ministries of Health and Health Canada.